SQSTM1 and familial pancreatic carcinoma: Immunoblotting results revealed that p62/SQSTM1 levels increased in a concentration- and time-dependent manner in IMB-6G-treated MiaPaCa-2 and HupT-3 cells (Fig. 2c, middle panel), indicating IMB-6G induced accumulation of autophagosomes reflects an inhibition of their degradation in pancreatic cancer cells.