We again identified TF–HPV status associations by t-test and found a similar set of TFs (23 out of 33) whose activities significantly differed between HPV(+) and HPV(−) tumours; the 33 identified TFs were also enriched among the top-ranked TFs in the new cohort (P<10−5, Mann–Whitney test) (Fig. 3c and Supplementary Table 6). This evidence concerns the gene TF and neoplasm.