To test whether the crtB marker could be extended to track virus infection in cucurbits, we constructed two recombinant clones of Zucchini yellow mosaic virus (ZYMV; genus Potyvirus, family Potyviridae) that harbor the sequences, which encode either crtB (ZYMV-crtB) or Rosea1 (ZYMV-Ros1) (Fig. 5d and Supplementary Fig. S3). This evidence concerns the gene ROS1 and viral infectious disease.