Wound healing assays showed that the migration ability of HCC cells was significantly promoted by TGF-β1 treatment (Fig. 4d, Control vs. TGF-β1, p < 0.01, respectively) but repressed by Neferine treatment in TGF-β1-treated HCC cells (Fig. 4d, TGF-β1 + Neferine vs. TGF-β1, p < 0.001, respectively). This evidence concerns the gene TGFB1 and hepatocellular carcinoma.