GPT and cholestasis: Also at 24 h post-APAP, we observed characteristic centrilobular hepatic necrosis (Fig. 6) combined with highly elevated LFTs (ALT: 700–12820 U/L; n = 5) in mice treated with 150 mg/kg and 300 mg/kg APAP, with a 2–3x fold increase in Bilirubin and elevated Alkaline Phosphatase (ALP), hallmarks of AILI with cholestasis.