Next, to confirm that Jellyfish-HE-induced apoptosis is directly under the control of Jellyfish-HE, cells were treated with 40 μg/ml Jellyfish-HE for 8 h and then assessed using Annexin V-FITC/PI staining and quantitative analysis (Fig. 3C). Here, ANXA5 is linked to hereditary elliptocytosis.