These results indicate that a PI3K signaling is involved in the HG-regulated p27Kip1 expression in RMCs, and imply that the induction of PI3K-regulated p27Kip1 pathway may contribute to the accumulation of ECM during hyperglycemia; nevertheless, the further studies are needed to clarify this possibility of its therapeutic potential in the future. Here, CDKN1B is linked to Hyperglycemia.