HES1 and liver cancer: Western blotting (Figure 10) showed that at 48 h after transfection with miR-34a mimics, liver cancer Huh7 cells had down-regulated protein expressions of Notch signaling pathway-related Notch1, Notch4 and Hes1 and apoptosis-related Bcl-2 and Bcl-xL, but had up-regulated expressions of cell cycle-related P21 and apoptosis-related Bax when compared with the blank group and the NC group (all P < 0.05).