According to the results of qRT-PCR (Figure 9), the liver cancer Huh7 cells in the miR-34a inhibitor group had decreased expression of miR-34a and increased mRNA expressions of Notch1, Notch4 and Hes1 in comparison to the blank group and the NC group (all P < 0.05), while the cells in the miR-34a mimics group had increased expression of miR-34a and decreased mRNA expressions of Notch, Notch4 and Hes1 when compared with the blank group and the NC group (all P < 0.05). This evidence concerns the gene NOTCH4 and liver cancer.