In the present study such an acidic pH caused only minor changes in TRPM7 currents in the presence of extracellular divalents, suggesting that TRPM7 may not be a major contributor to the electrophysiological changes during acute ischemia in the heart, especially since the channel is also blocked by intracellular acidic pH and by the increased level of free Mg2+ following ischemia-associated ATP breakdown. This evidence concerns the gene TRPM7 and ischemia.