Keeping in mind all mentioned above, the objective of this study was to assess predictive value of tumor markers p2PSA and its derivates, %p2PSA, and PHI in detection of patients with aggressive PC (assumed as Gleason score ≥7) in a sub-cohort of men whose total PSA ranged from 2 to 10ng/mL. This evidence concerns the gene KLK3 and pachyonychia congenita.