While most of the scFvs showed lower binding with the c9orf72 ALS samples compared to the sporadic ALS samples, the ALS-TDP10 scFv was the only scFv that also showed significantly higher reactivity with the c9orf72 ALS samples compared to the control group (Fig. 6e). Here, C9orf72 is linked to amyotrophic lateral sclerosis.