To determine the significance of CBX6 expression in HCC, we first detected CBX6 mRNA and protein expression levels in a normal human liver cell line (THLE-3) and several human HCC cell lines (MHCC-97L, Huh7, HepG2, SMMC7721, HCCLM3, and MHCC-97H). The gene discussed is CBX6; the disease is hepatocellular carcinoma.