By analyzing the association between CBX6 protein levels and clinicopathological characteristics using TMA analysis, we found that high CBX6expression was more frequent in HCC patients with a larger tumor size (≥ 5 cm, p = 0.011) and multiple tumors (n ≥ 2, p = 0.018) than in patients with a smaller tumor size and single tumors, indicating that CBX6expression is closely correlated with tumor progression. The gene discussed is CBX6; the disease is hepatocellular carcinoma.