SMMC7721cells exhibited lower CBX6 expression and were thus selected to generate stable CBX6 over-expression cells (which we named SMMC-7721-CBX6), and HCCLM3 cells exhibited higher CBX6 expression and were thus selected to generate stable CBX6-knockdown cells (which we named HCCLM3-CBX6i).CBX6overexpression or knockdown efficiency was confirmed by qRT-PCR and western blotting (Supplementary Figure 2).Cell Counting Kit-8 (CCK8) was utilized to assess HCC cell proliferation in vitro. This evidence concerns the gene CBX6 and hepatocellular carcinoma.