Real-time quantitative polymerase chain reaction (qRT-PCR) and western blot analyses revealed that CBX6 mRNA and protein expression levels were both markedly increased in all six HCC cell lines compared to the THLE-3 cell line (Figure 1A).Then, we detected CBX6 mRNA expression in 50 pairs of primary HCC tissue samples and corresponding adjacent nontumor tissue samples. Here, CBX6 is linked to hepatocellular carcinoma.