To address this, we further investigated changes in corneal inflammation following depletion of corneal macrophages and found that at 18 h after corneal epithelial abrasion, injection of anti-CSF1R antibody significantly increased inflammation of the cornea, expression of inflammatory cytokines IL-1β and TNF-α, and the presence of neutrophils in the cornea to levels higher than in the isotype immunoglobulin G control group. This evidence concerns the gene TNF and inflammation.