Three patients carry mutations in metastatic samples not detected in the primary tumour samples: TCF7L2 (c.T631G, p.F211V, liver metastasis of patient D23), GNAS (c.G2531A, p.R844H in one of three lymph node metastases of patient D26), CARD11 (c.C1267T, p.R423W) and TP53 (c.A736G, p.M246V; Fig. 2b). This evidence concerns the gene TP53 and neoplasm.