Here, the soluble mediator gene expression profile of resting T1D-derived IEC cultures was similar to that observed in the whole tissue, whereby 5- and 3.6-fold higher mRNA levels of BD-2 and IL-17C, respectively, were observed in T1D-derived cultures, when compared to non-TID derived cultures (Figures 4A,B), without similar upregulation in immunoregulatory mediators such as TSLP or IL-10 (Figures 4A–C). Here, TSLP is linked to type 1 diabetes mellitus.