Recent studies have shown that Reactive oxygen species (ROS)/c-Jun NH2-terminal kinase (JNK) signal pathway plays a critical role in celastrol induced cell apoptosis; treatment of cancer cells with celastrol activates caspase-3, -8, -9, DR5, and cleavage of PARP, Bid, upregulates the expression of LC3B-II. The gene discussed is CASP3; the disease is cancer.