To determine if NFIB is required for the increased migration seen in melanoma cells over-expressing BRN2, A2058− BRN2, MM96L− BRN2, and HT144− BRN2 cells were transfected with siRNA pools targeting NFIB or BRN2 itself (Figs. 4A, 5B, S2A, S2B and S2D). This evidence concerns the gene NFIB and melanoma.