Conversely, constitutive BRN2 overexpression was also performed in these same cell lines and revealed a commensurate increase in both NFIB (3.8, 3.7, 2.4 fold respectively) and EZH2 (2.5, 2.3, 2.6 fold respectively) expression across all three melanoma cell lines (Fig. 2B). The gene discussed is EZH2; the disease is melanoma.