NFIB and melanoma: Within this dataset, a grey bar represents level of NFIB expression for each individual tumour, and the purple line represents a moving average of NFIB expression per 20 melanomas across the sample set (Fig. 5C) with a strong association between NFIB levels and expression of the invasive gene set evident (Spearman correlation P = 3.553 × 10− 15).