Modulation of NFIB expression induced a very similar migration phenotype to BRN2 whereby a significantly increased migratory capacity was seen in response to over-expression of NFIB within wound healing assays (Figs. 3H, S1B, S1D, S1F and S1H), while siRNA knock-down significantly decreased melanoma cell migration rates (Figs. 3G, S1A, S1C, S1E and S1G). This evidence concerns the gene NFIB and melanoma.