Immunostaining of NFIB within both mouse xenograft and human primary/metastatic melanoma tumours shows clear co-localization with BRN2 expressing populations, indicating that NFIB expression within melanoma tumours is most likely linked with these more undifferentiated and invasive cellular populations previously associated with BRN2 expression (Goodall et al., 2008, Pinner et al., 2009). This evidence concerns the gene POU3F2 and melanoma.