NFIB and melanoma: Firstly, siRNA knockdown of BRN2 in high NFIB expressing lines (A2058, MM96L and HT144) produced a considerable reduction in NFIB expression to levels of approximately 80% fold or less in A2058 and MM96L melanoma cells and a modest but consistent reduction in the HT144 cells (20%, 40% and 80%) across the three independent siRNA treatment groups (Fig. 2A).