These recently published outcomes, as well as the available literature data on the contribution of TLR2 SNPs to the occurrence of HCMV infection, prompted us to undertake further research, evaluating the role of TLR2 1350 T>C coding synonymous (Ser450, rs3804100), as well as 2029 C>T (Arg677Trp, rs121917864) and 2258 G>A non-synonymous (Arg753Gln) SNPs in the development of HCMV congenital infection in fetuses and neonates. This evidence concerns the gene TLR2 and cytomegalovirus infection.