Taking into account the outcomes for TLR2 2258 G>A SNP, obtained in the current study, as well as for TLR4 896 A>G, 1196 C>T and TLR9 2848 G>A SNPs, reported in our previous paper [14], the additional multiple-SNP analysis showed the occurrence of complex AA variants in the range of both TLR2 and TLR9 polymorphisms to be correlated with an increased risk of HCMV infection among studied fetuses and neonates (OR 11.58, 95% CI 1.19–112.59; P ≤ 0.050, see Table 4). This evidence concerns the gene TLR2 and cytomegalovirus infection.