Using Δβ > 0.17, we identified 21,925 DMLs between ER+ and ER– breast tumors in the FF breast tumors included in this study and 13,594 DMLs in the FFPE slide, 11,764 DMLs in the FFPE punch and 11,960 DMLs in FFPE curl breast tumors. Here, ESR1 is linked to breast neoplasm.