TNF and Chagas disease: In this work, we evaluated the intracellular levels of MMP-2, MMP-9 and the cytokines IL-1β, TNF-α, TGF-β and IL-10 in neutrophils and monocytes from patients with IND and CARD clinical forms of Chagas disease and non-infected (NI) individuals, before (ex vivo condition) and after in vitro stimulation with T. cruzi derived antigens.