Importantly, while CDCA alone had no effects on markers of liver fibrosis including αSMA, Col1α1 and TGFβ1 and worsened the severity of liver damage caused by CCl4, the combination of CDCA with ISO-COOH, resulted in a significant anti-fibrotic and anti-inflammatory activity. This evidence concerns the gene TGFB1 and Hepatic fibrosis.