In the present study, none of 32 E. coli strains expressed verocytotoxins (vt1 and vt2 genes) or eae. Furthermore, ipaH (EIEC), and eae, eaf, and bfp (EPEC), which are intimately associated with other pathotypes involved in human infections, were not identified in the 32 E. coli strains isolated from our diarrheic and nondiarrheic foals. This evidence concerns the gene RNF112 and infection.