To see if the lack of trp1 affects the development of oocysts, we counted the number of oocysts at different time points after infection of mosquitoes by trp1(-)mCh or the fluorescent control line fluo (Klug et al., 2016), which expresses mCherry under control of the CSP promoter and GFP under control of the ef1α promoter (Figure 2B). This evidence concerns the gene DNAJC5 and infection.