Among them, we found NEK2, RAD51AP1 and LYPD1 (LY6/PLAUR domain containing 1), a neuronal transmembrane protein potentially associated with brain metastases.36 Statistical analysis revealed that expression of the CD271-responsive genes NEK2 (P=1.56E–06), RAD51AP1 (P=2.67E–04), HMMR (P=5.47E–07) and TOP2A (P=1.56E–06) but not FGF13 (P=0.3122) significantly discriminated primary tumors and melanoma metastases (Figure 5f and Supplementary Figures S7B and C). This evidence concerns the gene TOP2A and melanoma.