CD4 and infection: To compare effector cell numbers in the skin, we co-transferred 5 × 104 congenically-marked HSV-specific CD4+ gDT-II and CD8+ gBT-I T cells into recipient mice before infection, administered anti-Ly6G depleting antibodies at Days 1, 3 and 5 p.i., prior to analysing the infected skin at Day 6 p.i. via flow cytometry (Fig. 5a).