Quantitative Real Time RT-PCR analysis of the reactive fibroblasts markers Acta2, Tagln as well as the tumor-promoting factors Vegf (vascular endothelial growth factor), Tgfb and Mmp2 (matrix metalloproteinase 2) in total RNA isolates of HS5 fibroblasts (+/−Cav1 and +/−XRT) further confirmed the more reactive phenotype of Cav1(−) HS5 fibroblasts (Fig. 7E). This evidence concerns the gene MMP2 and neoplasm.