Biomedical database and TargetScan (target point analysing tool) were employed, and the gene structures were analysed by gene complementation theory, verifying that CXCR4 was one of the target genes of miR-338 in bone cancer cells with the pre-experiment, and also that miRNA-338 seems to play a biological role by identifying and combining with CXCR4 mRNA. The gene discussed is CXCR4; the disease is bone cancer.