TWIST1 and hepatocellular carcinoma: To address whether NF-κB binds directly to the predicted sites in the SNAIL1, SLUG, TWIST1 and SIP1 promoters, we performed a ChIP assay followed by qPCR (ChIP-qPCR) using the MDA-MB-231 (Fig 7A), HCC-1954 (Fig 7B) and MCF-7 (S3C Fig) cell lines.