To support a possible role of Ust in melanoma, we tested three metastasizing human melanoma cell lines, MV3 [31], HT199 and HT168M [30] that all express UST. The biological relevance of the data obtained with mouse melanoma cells is supported by the human melanoma cell line MV3 which expresses UST. siRNA-mediated UST knock-down in MV3 cells also showed a reduction in ITGa5 and adhesion. This evidence concerns the gene UST and melanoma.