NKD1 and posterior cortical atrophy: To provide the further evidence that NKD1 is a direct downstream mediator of miR-744, we first detected the expression of NKD1 between these PCa cells and found that the expression of NKD1 in PC3 and DU145 cells were less than LNCaP cells, then we conducted the siRNA-mediated NKD1 knockdown experiments on LNCaP cells (Figure 5A and 5B).