Since ATA suppressed TWEAK stimulation of NF-κB activity in HEK293 cells, we next investigated the effects of ATA on signaling cascades downstream of Fn14 using two established glioma cell lines (T98G and A172) and the GBM patient-derived xenograft (PDX) line GBM44. This evidence concerns the gene TNFRSF12A and central nervous system cancer.