To specifically label the phospholipids of Dictyostelium prior to infection, we incubated wild type and dgat1&2 DKO cells overnight with exogenous FAs to induce lipid synthesis and storage and chased afterwards for 1 hour with Topfluor-Lysophosphatidylcholine (PC) complexed to defatted Bovine Serum Albumin (BSA). Here, ALB is linked to infection.