Conversely, overexpression of H19 significantly increases sphere-forming capacity.36 H19 also promotes soft-agar colony formation in breast cancer cells.37 Consistently, our studies demonstrated that BCSC-enriched populations (i.e., ALDH1+ subpopulation, mammoshperes, 3D culture cells and SP cells) and breast tumors displayed high lncRNA-H19 expression. This evidence concerns the gene ALDH1A1 and breast carcinoma.