To investigate the expression pattern of IL-35 in pancreatic carcinoma, we performed immunohistochemistry (IHC) staining to detect EBI3 and P35 co-expression in tumour tissues and the adjacent normal pancreas tissues (pancreatic tissues 2–3 cm around the tumour border; Supplementary Fig. 1) of five histopathological subtypes of pancreatic carcinoma (PDAC, mucinous carcinoma, giant cell carcinoma, malignant neuroendocrine carcinoma and acinic cell carcinoma). This evidence concerns the gene EBI3 and acinar cell carcinoma.