Interestingly, by analyzing the quantification of IHC staining and performing the Spearman's rank correlation coefficient test and linear tendency test, we found that TIM3 expression in HNSCC was significantly correlated with CD8 (P < 0.001, r = 0.3126), CD11b (P < 0.001, r = 0.3892), and CD33 (P < 0.001, r = 0.3089, Fig. 3B). This evidence concerns the gene ITGAM and head and neck squamous cell carcinoma.