SFRP1 and breast cancer: In contrast to the normal mammary epithelial cell line (MCF10A), BC cell lines (MDA-MB-231, BT-20, MCF-7 and T-47D) had lower mRNA and protein expressions of SFRP1, with the lowest being T-47D cell line (all P < 0.05) (Figure 3D, 3E, 3F).