To further explain the mechanism by which TFS facilitated apoptosis, we performed RT-PCR, Western blot and IHC assay to examine the mRNA and protein expressions of apoptosis-related proteins, namely, Bcl-2, Bax, Bcl-xL and XIAP, in transplanted CRC tumors with or without TFS treatment. The gene discussed is BCL2; the disease is colorectal carcinoma.