To further investigate the role of ACSS2 expression in acetate utilization for lipogenic AcCoA, we performed U-13C-acetate tracing in a set of six cancer cell lines originating from cervical (HeLa), lung (A549), pancreatic (AsPC-1), and breast tumors (MDA-MB-231, MDA-MB-468, and BT-474). Here, ACSS2 is linked to breast neoplasm.