CES2 expression led to widespread changes in phospholipids, lysophospholipids, and ethers lipids, which were comparable to those induced by high-fat diet feeding (Figures 6A and S3). Similar to our observations in human obesity, high-fat diet feeding robustly increased oleate-containing DAGs, and this effect was reversed by CES2 expression (Figure 6B). This evidence concerns the gene CES2 and obesity due to melanocortin 4 receptor deficiency.