To more clearly delineate the roles and the interconnections between HIF-1α-related metabolic changes and pH-regulating enzymes in tumor behavior, we analyzed the expression of HIF-1α, CAIX, MCT1 and MCT4 proteins and their associations with each other and with a miR-210/ICU signaling pathway in patient-derived primary SCCs. This evidence concerns the gene HIF1A and neoplasm.