PECAM1 and neoplasm: To confirm the TAMs nature of these cells, the macrophage specific marker CD31 was used as a marker for endothelial cells and monocyte-derived macrophages [17] and Iba1 (ionized calcium-binding adapter molecule 1) as a microglia/macrophage marker, commonly used to label total tumor associated macrophagies (TAMs) [18,19,20].