CTCFL and colorectal cancer: To confirm these observations, BORIS expression and sub-cellular localization were assessed in four colorectal cancer cell lines: a colorectal adenocarcinoma cell line (Caco-2) and 3 colorectal carcinoma cell lines (COLO 205, HT29, and HCT116), together with one normal colon-derived fibroblast cell line (CCD-18Co).