Unlike cancer-derived epithelial cell lines, organoids are derived from primary stem cells and consist of a heterogeneous cell population with cell-type specific markers which were unaltered by the presence of inulin or scFOS (Supplementary Fig. 1b).When grown on Transwell filter supports, the intestinal organoids form a two-dimensional monolayer with clear cell border demarcations and TJ (ZO-1) expression (Supplementary Fig. 1c). This evidence concerns the gene TJP1 and cancer.