Importantly, using this approach, shRNA screens have shown that loss of expression of methylthioadenosine phosphorylase (MTAP), an enzyme involved in the methionine salvage pathway, leads to sensitization to the knockdown of PRMT5, an enzyme involved in the methylosome, in a range of malignancies including melanoma, breast and lung cancer (Kryukov et al., 2016). This evidence concerns the gene MTAP and lung cancer.