TNF and airway hyperresponsiveness: 2012a,b b), TNF‐α pretreatment consistently increased the reactivity to 30 nmol/L U‐46619 when compared to control conditions. However, MAG‐DPA treatment (0.3 or 1 μmol/L) was able to reverse this overreactivity induced by TNF‐α. Hence, despite the presence of TNF‐α, the mean agonist‐induced tone in the presence of 1 μmol/L MAG‐DPA was close to the tone developed in control condition. Thus, μM concentrations of MAG‐DPA were able to blunt TNF‐α‐induced airway hyperresponsiveness to U‐46619.