DCT and infection: Here, we studied DCT’s potential role in HPV infection and found that DCT depletion (i) notably decreased infection in HaCaTs; (ii) did not impact viral binding and delivery of PsV to early endosomes; (iii) did not prevent TGN localization of PsV and EdU-labeled pseudogenome; (iv) increased ER localization of EdU-labeled pseudogenome; (v) resulted in increased ROS in HaCaTs; (vi) interfered with HPV infection in partially different ways compared to γ-secretase inhibition.