Utilizing our recently published NGS based 2GDMODY protocol(13), we have screened 50 pregnant women with diabetes for a comprehensive panel of 13 MODY genes [HNF1A, HNF4A, GCK, PDX1, HNF1B, NEUROD1, KLF11, CEL, PAX4, INS, BLK, ABCC8,KCNJ11]. This evidence concerns the gene PDX1 and diabetes mellitus.