In our study, to identify proteins with differential affinities to ALS-linked mutants than to FUS-WT (wild type), we performed co-immunoprecipitation (co-IP) of FLAG-tagged FUS-WT, FUS-R521C, and FUS-P525L, and liquid chromatography-mass spectrometry (LC-MS). The gene discussed is FUS; the disease is amyotrophic lateral sclerosis.