Since the levels of 1,25(OH)2D were high in CKD mice, we also measured 25(OH)D levels in the αKlotho and Fgfr1–4 groups: αKlothoflox/flox, control (64.6 ± 7.8 ng/mL, n = 5) vs αKlothoflox/flox, CKD (52.0 ± 4.4 ng/mL, n = 5), αKlothoflox/flox;PTH-Cre, control (60.4 ± 17.7 ng/mL, n = 5), αKlothoflox/flox;PTH-Cre, CKD (57.4 ± 13.9 ng/mL, n = 5); Fgfr1–4flox/flox, control(54.4 ± 7.6 ng/mL, n = 5) vs Fgfr1–4flox/flox, CKD (61.4 ± 20.6 ng/mL, n = 5), Fgfr1–4flox/flox;PTH-Cre, control (65.0 ± 12.5 ng/mL, n = 5), Fgfr1–4flox/flox;PTH-Cre, CKD (61.0 ± 8.1 ng/mL, n = 5). Here, PTH is linked to chronic kidney disease.