To test this, we first used MitoTracker dye and quantitatively analyzed uptake in the PTENWT melanoma cells using flow cytometry as an indication of the total amount of mitochondria in the cells, however, we did not detect a difference in cells either treated with rWNT3A or overexpressing WNT3A, compared with control-treated cells (Figure 6a). This evidence concerns the gene WNT3A and melanoma.