To confirm that these WNT/β-catenin-mediated effects on melanoma cell motility are PTEN dependent, we overexpressed PTEN in the mutant lines (A2058 and M229; Supplementary Figure 2d) and found that re-establishment of PTEN expression could the switch the effect of rWNT3A stimulation from pro- to anti-migratory (Figure 2f and Supplementary Figure 2e). Here, PTEN is linked to melanoma.