AKT1 and melanoma: PTEN is known to possess both PI3K-dependent and -independent activities.44 To investigate if the WNT/β-catenin-mediated effects on melanoma cell motility are PI3K dependent, we used the PI3K inhibitor, LY294002, in PTENMut cells at a concentration that could inhibit PI3K-AKT signaling (Figure 2g), but could not significantly alter basal cell migration (Figure 2h).