By monitoring population doublings over several weeks, we found that hyperactive WNT3A signaling resulted in reduced cell counts (Figure 1g), which is comparable with previous findings.15 Analysis of cell division showed no significant difference in G0/G1, S and G2/M phases of the cell cycle in response to overexpressed WNT3A in the melanoma cells (Supplementary Figure 1a), demonstrating that the differences in cell numbers during long-term proliferation assays identified in Figure 1g were not due to cell cycle arrest. This evidence concerns the gene WNT3A and melanoma.