WNT3A and melanoma: Furthermore, constitutive overexpression of WNT3A using a WNT3A-iresGFP reporter construct in two of the lines (one PTENWT line, A375; and one PTENMut line, A2058; Figure 1e) also led to enhanced signaling from this reporter construct (Figure 1f), demonstrating that WNT3A activates β-catenin signaling the selected melanoma panel.